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KMID : 0861020110260040095
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Korea Journal of Herbology
2011 Volume.26 No. 4 p.95 ~ p.99
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Antioxidant Activities and Inhibitory Effect on Oxidative DNA Damage of extracts from Abeliophylli distichi Folium
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Park Jae-Ho
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Abstract
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Objective: In this study, we demonstrate the protective effect on oxidative DNA damage of leaf extracts from
Abeliophylli distichi Folium via its antioxidant activity for the establishment of new value for the herbal
medicine.
Methods: Abeliophylli distichi Folium leaves were extracted with hot-water and ethylacetate (EtOAC). The
1,1-diphenyl-2- picrylhydrazyl (DPPH) radical and hydroxyl scavenging assay and Fe2+ chelating assay were
performed for antioxidative effect and φX-174 RF I DNA cleavage assay and intracellular DNA damage assay
were used for inhibitory effect of intracellular DNA damage.
Results: In DPPH, Hydroxyl radical scavenging activity and Fe2+ chelating activity of EtOAC extracts were
94.72%, 62.88%, 41.13%, and hot-water extracts were 88.86%, 56.7%, 37.4% at 200 ㎍/㎖, respectively. Also,
those extracts showed protective effect of DNA damage against the oxidative stress.
Conclusion: These results indicated that the leaf extracts of Abeliophylli distichi Folium can be used as a
natural antioxidants, which effectively inhibits the oxidative DNA damage.
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KEYWORD
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Abeliophylli distichi Folium, DPPH, Hydroxyl radical, chelating, DNA cleavage, DNA migration
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